5-(Iodoacetamido)fluorescein

Also known as: 5-IAF, Fluorescein-5-iodoacetamide, IAF, 5-(Iodoacetamido)fluorescein, 4(5)-(Iodoacetamido)fluorescein, 4-(iodoacetamido)fluorescein, N-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)-2-iodoacetamide, N-(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-yl)-2-iodoacetamide

5-(Iodoacetamido)fluorescein (CAS 63368-54-7) is a compound in the Organohalogen Compounds category listed in the Jingsj chemical database. It has the molecular formula C22H14INO6 and a molecular weight of 515.25400 g/mol. Key properties include a melting point of 265-267ºC (dec.)(lit.), a boiling point of 788.4ºC at 760 mmHg, a density of 1.95 g/cm3 and a flash point of 430.6ºC. No supplier offers are currently listed; request a quote. Record last updated Aug 21, 2026.

CAS No.: 63368-54-7
Jingsj CID: J-1958521-1
Molecular Formula: C₂₂H₁₄INO₆
Molecular Weight: 515.25400 g/mol
Category: Organohalogen Compounds
Last updated: 8/21/2026

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Physicochemical Properties

Melting Point	265-267ºC (dec.)(lit.)
Boiling Point	788.4ºC at 760 mmHg
Density	1.95 g/cm3
Flash Point	430.6ºC
Exact Mass	514.98700
PSA	116.84000
LogP	4.41510
Refractive Index	1.781

Activity & Biological Data

Additional Information	[1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374. [2]. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216(16):3533-7.

Additional Information

[1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374. [2]. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216(16):3533-7.

Frequently Asked Questions

What is the CAS number of 5-(Iodoacetamido)fluorescein?

The CAS number of 5-(Iodoacetamido)fluorescein is 63368-54-7.

What are the molecular formula and molecular weight of 5-(Iodoacetamido)fluorescein?

5-(Iodoacetamido)fluorescein has molecular formula C22H14INO6 and molecular weight 515.25400 g/mol.

Data compiled from public chemical databases such as PubChem and supplier catalogs; supplier offers are provided by suppliers.

Last updated: 8/21/2026

How to cite: Jingsj. "5-(Iodoacetamido)fluorescein (CAS 63368-54-7)". Jingsj Chemical Database. https://www.jingsj.com/compound/J-1958521-1 (accessed 2026-11-06).

Data for reference only; verify product details with suppliers before purchase.