Terminal deoxynucleotidyl transferase

Also known as: TdT, Terminal addition enzyme, Terminal transferase, DNA nucleotidylexotransferase, EC 2.7.7.31, Nucleoside triphosphate:DNA deoxynucleotidyl-exotransferase

Terminal deoxynucleotidyl transferase (CAS 9027-67-2) is a compound in the Organic Acids category listed in the Jingsj chemical database. It has the molecular formula C4H10S3 and a molecular weight of 154.325 g/mol. No supplier offers are currently listed; request a quote. Record last updated Aug 21, 2026.

CAS No.: 9027-67-2
Jingsj CID: J-51984-7
Molecular Formula: C₄H₁₀S₃
Molecular Weight: 154.325 g/mol
Category: Organic Acids
Last updated: 8/21/2026

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Physicochemical Properties

Stability	Analysis Note Activity assay: 200 mM potassium cacodylate, pH 7.2, 4 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM 3H-dATP, 70 μM d(pT)6, 37 °C. Biochem/physiol Actions Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3′-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3′ overhangs, but procedures have been developed to label blunt ends or 3′-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present. Each reaction mixture needs to be optimized. The enzyme is commonly used to add complementary homopolymer tails to vector and insert DNA for construction of recombinant DNA in vitro. Features and Benefits • Add homopolymers to vectors, inserts and cDNA for cloning • Label the 3′-end of double- and single-stranded DNA with non-radioactive and radioactive labels • Carry out in vitro mutagenesis by adding single nucleotides to DNA • Use in TUNEL assays with deoxyuridine triphosphate (dUTP) conjugated with a fluorescent chromophore or biotin to label nicked DNA in apoptotic cells Unit Definition One unit will incorporate 1 nanomole of dATP into acid-precipitable material in one hour at 37 °C using d(pT)6 as primer. Physical form Solution in 50 mM potassium phosphate, pH 7.4, 1 mM 2-mercaptoethanol and 50% glycerol (v/v)

Stability

Analysis Note Activity assay: 200 mM potassium cacodylate, pH 7.2, 4 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM 3H-dATP, 70 μM d(pT)6, 37 °C. Biochem/physiol Actions Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3′-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3′ overhangs, but procedures have been developed to label blunt ends or 3′-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present. Each reaction mixture needs to be optimized. The enzyme is commonly used to add complementary homopolymer tails to vector and insert DNA for construction of recombinant DNA in vitro. Features and Benefits • Add homopolymers to vectors, inserts and cDNA for cloning • Label the 3′-end of double- and single-stranded DNA with non-radioactive and radioactive labels • Carry out in vitro mutagenesis by adding single nucleotides to DNA • Use in TUNEL assays with deoxyuridine triphosphate (dUTP) conjugated with a fluorescent chromophore or biotin to label nicked DNA in apoptotic cells Unit Definition One unit will incorporate 1 nanomole of dATP into acid-precipitable material in one hour at 37 °C using d(pT)6 as primer. Physical form Solution in 50 mM potassium phosphate, pH 7.4, 1 mM 2-mercaptoethanol and 50% glycerol (v/v)

Activity & Biological Data

Additional Information	[1]. Boulé JB, et, al. Terminal deoxynucleotidyl transferase indiscriminately incorporates ribonucleotides and deoxyribonucleotides. J Biol Chem. 2001 Aug 17;276(33):31388-93.

Frequently Asked Questions

What is the CAS number of Terminal deoxynucleotidyl transferase?

The CAS number of Terminal deoxynucleotidyl transferase is 9027-67-2.

What are the molecular formula and molecular weight of Terminal deoxynucleotidyl transferase?

Terminal deoxynucleotidyl transferase has molecular formula C4H10S3 and molecular weight 154.325 g/mol.

Data compiled from public chemical databases such as PubChem and supplier catalogs; supplier offers are provided by suppliers.

Last updated: 8/21/2026

How to cite: Jingsj. "Terminal deoxynucleotidyl transferase (CAS 9027-67-2)". Jingsj Chemical Database. https://www.jingsj.com/compound/J-51984-7 (accessed 2026-11-06).

Data for reference only; verify product details with suppliers before purchase.