Dihydrofluorescein diacetate

Also known as: Diacetyldihydrofluorescein, 2-(3,6-Diacetoxy-9H-xanthen-9-yl)benzoic acid, 2-(3,6-Diacetyloxy-9H-xanthen-9-yl)benzoic acid, 2-[3,6-Bis(acetyloxy)-9H-xanthen-9-yl]benzoic acid, Diacetyldihydrofluorescein [for detection of alcohols and amines], MFCD00005056

Dihydrofluorescein diacetate (CAS 35340-49-9) is a compound in the Organic Acids category listed in the Jingsj chemical database. It has the molecular formula C24H18O7 and a molecular weight of 418.39600 g/mol. Key properties include a melting point of 213-215ºC(lit.), a boiling point of 554.3ºC at 760 mmHg and a density of 1.353g/cm3. No supplier offers are currently listed; request a quote. Record last updated Aug 21, 2026.

CAS No.: 35340-49-9
Jingsj CID: J-46535-0
Molecular Formula: C₂₄H₁₈O₇
Molecular Weight: 418.39600 g/mol
Category: Organic Acids
Last updated: 8/21/2026

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Physicochemical Properties

Melting Point	213-215ºC(lit.)
Boiling Point	554.3ºC at 760 mmHg
Density	1.353g/cm3
Exact Mass	418.10500
PSA	99.13000
LogP	4.52130
Refractive Index	1.626

Activity & Biological Data

Additional Information	[1]. Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med. 1999 Jul;27(1-2):146-59. [2]. Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003 Oct;120(4):319-25. [3]. Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63.

Additional Information

[1]. Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med. 1999 Jul;27(1-2):146-59. [2]. Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003 Oct;120(4):319-25. [3]. Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63.

Customs & Trade Information

HS Code	2932999099
Summary	2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

Frequently Asked Questions

What is the CAS number of Dihydrofluorescein diacetate?

The CAS number of Dihydrofluorescein diacetate is 35340-49-9.

What are the molecular formula and molecular weight of Dihydrofluorescein diacetate?

Dihydrofluorescein diacetate has molecular formula C24H18O7 and molecular weight 418.39600 g/mol.

What are the upstream and downstream products of Dihydrofluorescein diacetate?

For Dihydrofluorescein diacetate, Upstream Products: Fluorescin; Downstream Products: Fluorescin.

Data compiled from public chemical databases such as PubChem and supplier catalogs; supplier offers are provided by suppliers.

Last updated: 8/21/2026

How to cite: Jingsj. "Dihydrofluorescein diacetate (CAS 35340-49-9)". Jingsj Chemical Database. https://www.jingsj.com/compound/J-46535-0 (accessed 2026-11-09).

Data for reference only; verify product details with suppliers before purchase.