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2',7'-Dichlorofluorescein

Also known as: 2',7'-Dichloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one, 2',7'-dichloro-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one, 2',7'-Dichloro-3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one, Dichlorofluorescein

2',7'-Dichlorofluorescein (CAS 76-54-0) is a compound in the Organohalogen Compounds category listed in the Jingsj chemical database. It has the molecular formula C20H10Cl2O5 and a molecular weight of 401.196 g/mol. Key properties include a melting point of 280 °C (dec.)(lit.), a boiling point of 634.4±55.0 °C at 760 mmHg, a density of 1.8±0.1 g/cm3 and a flash point of 337.4±31.5 °C. It is classified under GHS with signal word Danger and hazard statements H225 — Highly flammable liquid and vapour, H319 — Causes serious eye irritation and H336 — May cause drowsiness or dizziness. No supplier offers are currently listed; request a quote. Record last updated Aug 21, 2026.

CAS No.
76-54-0
Jingsj CID
J-30105-3
Molecular Formula
C20H10Cl2O5
Molecular Weight
401.196 g/mol
Category
Organohalogen Compounds
Last updated
8/21/2026

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Physicochemical Properties

Melting Point280 °C (dec.)(lit.)
Boiling Point634.4±55.0 °C at 760 mmHg
Density1.8±0.1 g/cm3
Flash Point337.4±31.5 °C
Exact Mass399.990540
PSA75.99000
LogP4.10
Vapor Pressure0.0±1.9 mmHg at 25°C
Refractive Index1.797
StabilityStable. Incompatible with strong oxidizing agents.

GHS Safety & Compliance

Flammable / IrritantSignal word: Danger
  • H225 — Highly flammable liquid and vapour
  • H319 — Causes serious eye irritation
  • H336 — May cause drowsiness or dizziness

Activity & Biological Data

Additional Information[1]. Reiniers MJ, et al. Preparation and Practical Applications of 2',7'-Dichlorodihydrofluorescein in Redox Assays. Anal Chem. 2017 Apr 4;89(7):3853-3857. [2]. Xiuping Chen, et al. 2',7'-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy. Free Radic Res. 2010 Jun;44(6):587-604.

Customs & Trade Information

HS Code2932999099
Summary2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

Frequently Asked Questions

What is the CAS number of 2',7'-Dichlorofluorescein?

The CAS number of 2',7'-Dichlorofluorescein is 76-54-0.

What are the molecular formula and molecular weight of 2',7'-Dichlorofluorescein?

2',7'-Dichlorofluorescein has molecular formula C20H10Cl2O5 and molecular weight 401.196 g/mol.

Is 2',7'-Dichlorofluorescein hazardous?

2',7'-Dichlorofluorescein has GHS signal word Danger and hazard statements H225 — Highly flammable liquid and vapour; H319 — Causes serious eye irritation; H336 — May cause drowsiness or dizziness.

What are the upstream and downstream products of 2',7'-Dichlorofluorescein?

For 2',7'-Dichlorofluorescein, Upstream Products: 2',7'-Dichlorodihydrofluorescein diacetate, Phthalic anhydride, 4-Chlororesorcinol; Downstream Products: Xanthene, 2',7'-Dichlorofluorescein diacetate.

Data compiled from public chemical databases such as PubChem and supplier catalogs; supplier offers are provided by suppliers.

Last updated:

How to cite: Jingsj. "2',7'-Dichlorofluorescein (CAS 76-54-0)". Jingsj Chemical Database. https://www.jingsj.com/compound/J-30105-3 (accessed 2026-11-09).

Data for reference only; verify product details with suppliers before purchase.