商品名：二(2-羟乙基)亚氨基三(羟甲基)甲烷(Bis-tris)

别 名：双(2-羟乙基)氨基(三羟甲基)甲烷，2,2-双二羟甲基苯胺
2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol
2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
Bis-tris

英文名：BIS-TRIS

产 地：湖南

Cas号：6976-37-0

MDL：MFCD00002853

Beilstein：2205275

EINECS：230-237-7

分子结构：

分子式：C8H19NO5

分子量：209.2400

备注：

性状：溶于水、乙醇以及有机溶剂。
熔点：103-104℃
pH range 5.8 - 7.2
pKa (25 °C) 6.5

质量标准：98%:

外观Appearance                          白色或无色结晶
红外光谱鉴定Infrared spectrometry       和对照品匹配
纯度Purity by Titration by H2SO4        ≥99.0%
纯度Purity(HPLC)                        ≥98%
熔点Melting point                       102°C - 106°C
水不溶物                                ≤0.01%
重金属Heavy metals (as Pb):             ≤0.0003 %  
pH (1 %; H2O):                          8.8 - 9.6
等电点pKa 25℃                          6.45-6.65  
干燥失重Water:                          ≤1 %  
铁Fe:                                   ≤0.0005 %  
吸光度A (1 cm/0.1 M in water):  
280 nm:                                 ≤0.04  
340 nm:                                 ≤0.02  


品级Grade                           细胞与昆虫细胞培养级, ≥98% (HPLC))
                   (for cell culture, for insect cell culture, ≥98% (HPLC))
外观Appearance                          White Crystalline Powder
红外光谱Infrared spectrum               Conforms to Structure
纯度Purity by Titration by H2SO4        ≥99.0%
纯度Purity(HPLC)                        ≥98%
水溶性Solubility                        Colorless to Very Faint Yellow， Clear，500 mg/ml in H2O
水分Water (by Karl Fischer)             ≤1 %
重金属Heavy Metals                      ≤ 5 ppm
DNA酶DNAse, Exonuclease Detection       None Detected
内切酶NICKase,Endonuclease Detection    None Detected
RNA酶RNAse Detection                    None Detected
蛋白酶Protease Detection                None Detected
内毒素Endotoxin Level                  1 EU/mg

存储：常温

用途：缓冲液，用于检测肌酸肌酶；也用于在冷冻干燥中有效保护血红蛋白；阴极电解液，用在IEF/2-D凝胶电泳。

联合编号：

RISK：

Safety：

Hazard：