商品名：1,4-哌嗪二乙磺酸(PIPES)
别 名：哌嗪-N,N＇-二(2-乙磺酸)，哌嗪-1,4-二乙磺酸
1,4-Piperazinediethanesulfonic acid
1,4-Piperazinediethanesulfonic acid
Piperazine-1,4-bis(2-ethanesulfonic acid)
Piperazine-N,N′-bis(2-ethanesulfonic acid)
英文名：PIPES
产 地：湖南
Cas号：5625-37-6
MDL：MFCD00006159
Beilstein：817713
EINECS：227-057-6
分子结构：
分子式：C8H18N2O6S2
分子量：302.3700
备注：
性状：白色粉末。
pH range 6.1 - 7.5
pKa (25 °C) 6.8
熔点：＞300℃
质量标准：99%：
外观Appearance white crystalline powder
红外光谱Infrared spectrometry Conforms to Structure
纯度Purity(by sulfuric acid titration) ≥99.0%
UV absorbance ≤0.1 at 290nm (20%w/w in 1N NaOH)
水分Water (by Karl Fischer) ≤3%
重金属heavy metals (as lead) ≤ 5ppm
溶解性solubility clear colorless solution at 20g plus 80ml of 1N NaOH
99.5%,超纯级:
灼烧残渣Residue on ignition ≤0.2% (as SO4)
loss on drying, 110 °C ≤0.2%
useful pH range 6.1 - 7.5
pKa (25 °C) 6.8
碱溶性solubility 0.1 M NaOH: soluble0.1 M at 20 °C, clear, colorless
anion traces
氯chloride (Cl-): ≤200 ppm
硫酸根离子sulfate (SO42-): ≤50 ppm
cation traces Al: ≤5 ppm
As: ≤0.1 ppm
Ba: ≤5 ppm
Bi: ≤5 ppm
Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Li: ≤5 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Mo: ≤5 ppm
Na: ≤100 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Sr: ≤5 ppm
Zn: ≤5 ppm
λ 0.1 M in 1 M NaOH
UV absorption λ: 260 nm A: ≤0.055
λ: 280 nm A: ≤0.040
品级Grade 细胞培养级
(for cell culture, ≥99%)
外观Appearance white crystalline powder
红外光谱Infrared spectrometry Conforms to Structure
纯度Purity(by sulfuric acid titration) ≥99.0%
UV absorbance ≤0.1 at 290nm (20%w/w in 1N NaOH)
水分Water (by Karl Fischer) ≤3%
重金属heavy metals (as lead) ≤ 5ppm
溶解性solubility clear colorless solution at 20g plus 80ml of 1N NaOH
RNASE, DNASE, NICKASE AND PROTEASE none detected
内毒素endotoxin assay NMT 0.1 EU/MG
标准平板细菌计数standard plate count NMT 100 CFU/G
细胞培养测试cell culture test pass
品级Grade 分子生物学级
(for molecular biology,≥99.5% (T))
DNA酶DNases, none detected
RND酶RNases, none detected
不溶物insoluble matter, passes filter test
磷酸酶phosphatases, none detected
蛋白酶proteases, none detected
灼烧残渣Residue on ignition ≤0.2% (as SO4)
loss on drying, 110 °C ≤0.2%
useful pH range 6.1 - 7.5
pKa (25 °C) 6.8
碱溶性solubility 0.1 M NaOH: soluble0.1 M at 20 °C, clear, colorless
anion traces
氯chloride (Cl-): ≤200 ppm
硫酸根离子sulfate (SO42-): ≤50 ppm
cation traces Al: ≤5 ppm
As: ≤0.1 ppm
Ba: ≤5 ppm
Bi: ≤5 ppm
Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Li: ≤5 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Mo: ≤5 ppm
Na: ≤100 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Sr: ≤5 ppm
Zn: ≤5 ppm
λ 0.1 M in 1 M NaOH
UV absorption λ: 260 nm A: ≤0.055
λ: 280 nm A: ≤0.040
存储：室温
用途：mRNA的杂交选择 -预杂交缓冲液组分。
联合编号：
RISK：
Safety：
Hazard：