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无色透明液, 有水果香，易挥发, 对空气敏感, 能吸水分，水分能使其缓慢分解而呈酸性反应。能与氯仿、醇、丙酮和醚混溶，溶于水（10%ml/ml)。能溶解某些金属盐类（如氯化锂、氯化钴、氯化锌、氯化铁等。易燃。蒸气能与空气形成爆炸性混合物。半数致死量（大鼠，经口）11.3ml/kg。有刺激性。
密度：0.902
熔点：-83℃
沸点：77℃
折光率n20/D ：1.3719
闪点：-3 °C

GB/T 12589-90
项目Item                                  分析纯      化学纯
                                           (AR)        (CP)
含量(C₄H₈O₂)Assay,%                        99.0        97.0
密度(20℃)Density,g/mL                 0.899-0.901  0.897-0.901
色度Color，黑曾单位                      ≤ 10          20
蒸发残渣Evaporation residue,%            ≤ 0.0005     0.001
水份(H₂O)Water,%                         ≤ 0.1         0.4
酸度(以H⁺计)Acidity,mmol/100g            ≤ 0.08       0.08
甲醇(以CH₃OH计)Methanol,%                ≤ 0.1         0.2
乙醇(CH₃CH₂OH)Ethanol,%                  ≤ 0.2         0.5
乙酸甲酯(CH₃COOCH₃)Methyl formate,%      ≤ 0.1         0.3
易炭化物质Readily carbonizable substances   合格        合格


品级Grade                                 优级纯  
                                           (GR)
外观Appearance                           无色液体
红外光谱鉴别Infrared spectrometry        符合
纯度Purity                               ≥99.5% (GC)
水分Water                                
<0.05 % >蒸发残渣Residue after evaporation        
<0.001 % >亚硫酸变暗物Subst. darkened by H2SO4    
<10 APHA >甲醇Methanol                            
<0.1 % >乙醇Ethanol                              
<0.1 >乙酸甲酯Methyl acetate                  
<0.1 % >    

ACS级
项目名称　                                       ACS Grade
含量Assay(CH₃COOCH₂CH₃)                        ≥99.5%
色度Color(APHA)                                  10
灼烧残渣Residue after ignition                 ≤0.003%
水分（H₂O）Water                               ≤0.2%
可滴定酸Titrable acid                          ≤0.0009meq/g
硫酸变黑物substances darkened by sulfuric acid   Passes test


品级Grade                                   色谱纯  
                                          (for HPLC, ≥99.8%)
外观Appearance                              无色液体
红外光谱鉴别Infrared spectrometry           符合
纯度Purity                                  ≥99.8% (GC)  
水分water                                  
<0.050% (by Karl Fischer) >蒸发残渣Residue on Evaporation              
<0.0003% >λ H2O reference
UV absorption
  λ: 254 nm A:                              ≤1.00
  λ: 263 nm A:                              ≤0.05
  λ: 275 nm A:                              ≤0.01
  λ: 400 nm A:                              ≤0.05  


项目                                      光谱纯    
                                     (for spectroscopy)
外观Appearance                           无色液体
色度Color scale                          
<10 APHA >纯度Purity                               ≥99.0% (GC)
水分Water                                
<0.05 % >紫外吸收值UV：
  255 nm A:                              
<1 >  275 nm A:                              
<0.05 >  300 nm A:                              
<0.03 >  325 nm A:                              
<0.01 >蒸发残渣Residue on Evaporation            
<0.0003 % >

品级Grade                               蛋白测序级  
                                (for protein sequence analysis)
外观Appearance                          无色液体
红外光谱鉴别Infrared spectrometry       Conforms
纯度Purity                              ≥99.5% (GC)  
水分water                               ≤0.1%  
测序分析实验sequence analysis test      Pass


项目                                      农残级    
                                     (for spectroscopy)
外观Appearance                           无色液体
色度Color scale                          
<10 APHA >纯度Purity                               ≥99.5% (GC)
水分Water                                
<0.03 % >蒸发残渣Residue on Evaporation          
<0.0001 % >halogenated residue (GC/ECD)            
<10 ng/L (as heptachlor epoxide) >


品级Grade                               LC-MS级  
                              (for LC-MS, ≥99.9% (GC))
含量assay                               ≥99.9% (GC)
游离碱free alkali (as NH3)              ≤0.0005%
非挥发物non-volatile matter             ≤0.0005%
水分water                               ≤0.03%  (Karl Fischer)
透光度transmittance
  260 nm,                               ≤50%
  300 nm,                               ≤98%
Ca:                                     ≤0.1 ppm
K:                                      ≤0.1 ppm
Mg:                                     ≤0.1 ppm
Na:                                     ≤0.1 ppm
Test for suitability for LC-MS          passes



品级Grade                               生物工程级
                                    (biotech. grade, ≥99.9%)
外观Appearance                          Colorless Liquid
红外光谱鉴别Infrared spectrometry       Conforms
纯度Purity                              ≥99.9% (GC)
可滴定酸Titratable acid                 ≤0.0009 meq
水分Water                               ≤0.01 % (by Karl Fischer)
蒸发残渣Residue on Evaporation          ≤0.0003 %
λ H2O reference
UV absorption
  λ: 254 nm A:                          ≤1.00
  λ: 263 nm A:                          ≤0.05
  λ: 275 nm A:                          ≤0.01
  λ: 400 nm A:                          ≤0.01