99%:
外观Appearance White Powder or crystal
红外光谱Infrared spectrometry Conforms to Structure
熔点Melting point 263°C - 264°C
纯度Purity 99.0 - 101.0 % (以干基计)
干燥损失Loss on drying
<0.3 % (105°C, 3 小时) >重金属Heavy metals (as Pb)
<10 ppm >硫酸盐灰分Sulfated ash
<0.1 % >比旋度Specific rotation [a]20/D +20.5° - +21.5° (c=8, 6 N HCl)
痕量分析Trace analysis :
硫酸根SO4 < 200 PPM
氨基NH4 < 200 PPM
砷As < 1 PPM
铁Fe < 30 PPM
透光度Transmittance >95 % (c=10, H2O, 430 nm) 1 cm cell
其它氨基酸Foreign amino acids
<0.5 % (TLC, 50 ug 点样) >
Ultra pure,≥99.5% (AT):
外观Appearance White Powder or crystal
红外光谱Infrared spectrometry Conforms to Structure
纯度Purity ≥99.5% (AT)
比旋光度Specific rotation [α]20/D +20.5±0.5°, c = 5% in 5 M HCl
不溶物insoluble matter, passes filter test
外源氨基酸foreign amino acids ≤0.3%
灼烧残渣Residue on ignition ≤0.1% (as SO4)
干燥损失loss on drying, 20 °C (HV) ≤0.5%
pH 5.0-6.0 (25 °C, 0.5 M in H2O)
水溶性solubility H2O: soluble0.5 M at 20 °C, clear, colorless
硫酸根sulfate (SO42-): ≤100 ppm
Al: ≤5 ppm
As: ≤0.1 ppm
Ba: ≤5 ppm
Bi: ≤5 ppm
Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Li: ≤5 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Mo: ≤5 ppm
NH4+: ≤100 ppm
Na: ≤50 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Sr: ≤5 ppm
Zn: ≤5 ppm
λ 0.5 M in H2O
UV absorption λ: 260 nm A: ≤0.1
λ: 280 nm A: ≤0.1
品级Grade 非动物源,EP, JP, USP ;用于细胞培养,98.5 to 101.0%
(from non-animal source, EP, JP, USP, for cell culture, 98.5 to 101.0%)
来源origin non-animal source
外观appearance white crystalline powder
红外鉴别试验identification by ir compares to reference standard
水溶解性solubility clear colorless solution at 1.0g to 10ml of water
比旋光度specific rotation +20.8 to +21.5 deg (20degc/EP)
+20.4 to +21.4 deg (25degc/USP)
c = 8 in 6m hydrochloric acid
氯chloride 19.12 to 19.51%
铵ammonium ≤ 0.02%
硫酸盐sulfate ≤ 0.020%
铁iron ≤ 10 ppm
重金属heavy metals ≤ 10 ppm
砷arsenic ≤ 1 ppm
干燥失重loss on drying ≤ 0.10%
灼烧残渣residue on ignition ≤ 0.10%
残留溶剂residual solvents meets the requirements
茚三酮阳性物质ninhydrin-positive substances (other amino acids)
conforms
ph test 5.0 to 6.0 (10% in water)
含量assay (dry basis) 98.5 to 100.5%
内毒素endotoxin assay ≤ 1 eu/mg
细胞培养测试cell culture test pass
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